The TUNEL (TdT-mediated dUTP Nick End Labeling) Apoptosis Detection Kit can be used to detect apoptosis (programmed cell death) in individual cells.
During cell apoptosis, cells activate several DNA endonucleases, which cleave genomic DNA between nucleosomes. When DNA is extracted from apoptotic cells and examined by electrophoresis, a 180-200 bp DNA ladder is observed.
The TUNEL (TdT-mediated dUTP Nick End Labeling) Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation in tissue cells during the late stages of apoptosis. The assay utilizes the incorporation of Fluor 640-12-dUTP into the 3´-hydroxyl (3´-OH) ends of genomic DNA fragments, exposed by the action of terminal deoxynucleotidyl transferase (TdT), allowing detection using fluorescence microscopy or flow cytometry. Fluor 640 is a red fluorescent dye with high stability and strong brightness.
| 产品名称 | TUNEL Apoptosis Detection Kit |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0036 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 20 T, 50 T, 100 T |
| 储存条件 | -20 ℃ protect from light. |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
