Trypan blue is an acidic blue dye can stain dead cells by penetrating the cell membranes, researchers can distinguish live cells by this color change.
Trypan blue is an azo-based, hydrophilic, acidic blue dye that can penetrate the cell membranes of dead and dying cells, staining them blue. Live cells, due to the integrity of their cell membranes, exclude trypan blue. Therefore, live cells (which appear transparent and colorless) can be distinguished from dead cells by this color change. Based on this principle, trypan blue is widely used in routine assessments of cell viability.
Trypan Blue solution is used as a cell stain to assess cell viability using the dye exclusion test, where viable cells do not take up the dye. Trypan blue can also stain collagen and amyloid proteins. The complexes formed by trypan blue and protein emit red fluorescence. Furthermore, trypan blue (at appropriate concentrations) is often used to quench cell surface autofluorescence or other fluorescent signals.
| 产品名称 | 0.4% Trypan Blue Solution |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0031 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 20 mL, 100 mL |
| 储存条件 | Room temperature |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
