Hygromycin B

Hygro B is an antibiotic for selection of prokaryotic and eukaryotic cells expressing hygromycin resistance gene in molecular biology applications.

Hygromycin B is an aminoglycoside antibiotic that inhibits protein synthesis by disrupting translational fidelity and causing misreading of mRNA in both prokaryotic and eukaryotic cells. It is widely employed as a selective agent in molecular biology for the screening and maintenance of cells expressing the hygromycin resistance gene (hph or hygR). Effective against bacteria, fungi, and mammalian cells, hygromycin B is commonly used to establish stable transgenic cell lines, select genetically engineered microorganisms, and enrich edited populations in CRISPR-Cas9 systems. With high solubility and stability in culture media, it provides consistent and selective pressure in vitro and in vivo, making it essential for genetic engineering, antimicrobial studies, and long-term cell culture applications.

Hygromycin B is extensively used as a selective antibiotic in molecular and cellular biology for stable cell line development and maintenance. It enables efficient selection of eukaryotic and prokaryotic cells expressing the hygromycin resistance gene (hph/hygR), making it ideal for generating transgenic cell lines, CRISPR-edited clones, and recombinant microbial strains. Applied in both in vitro and in vivo systems, it supports long-term culture under selective pressure and is widely used in gene function validation, high-throughput screening, and antimicrobial mechanism studies. The antibiotic is effective across diverse cell types, including mammalian, insect, plant, and bacterial cells.

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