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HighQC™ Human (Parental) Head And Neck Cancer Stem Cell 1 vial

HighQC™ Human (Parental) Head And Neck Cancer Stem Cell

目录编号 ABC-SC0132 | 产品线：干细胞 | 种属：Human

Human (Parental) Head AndNeck Cancer Stem Cell; Frozen Vial and Plated cells are available.

HighQC™ Human (Parental) Head And Neck Cancer Stem Cells are tumor-initiating cells derived from human head and neck cancer tissues. Morphologically, the cells display a small, round to spindle-like appearance and grow as adherent monolayers under defined serum-free conditions. They express characteristic cancer stem cell markers such as CD44 and CD133, which are strongly associated with tumorigenic potential and therapy resistance. These cells exhibit stem-like properties, including self-renewal, the ability to differentiate into heterogeneous tumor cell populations and various epithelial cell types present in head and neck malignancies, and demonstrate high tumorigenicity in vivo. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.

HighQC™ Human (Parental) Head And Neck Cancer Stem Cell, Human Parental HNC CSC, hPara-HNCSC, Human Head Neck Cancer Progenitors

Additional Information

Growth ModeAdherent
Shipping InfoDry Ice
Biosafety Level1
Disease ModelHead and Neck Cancer
SynonymsHighQC™ Human (Parental) Head And Neck Cancer Stem Cell, Human Parental HNC CSC, hPara-HNCSC, Human Head Neck Cancer Progenitors

干细胞 1 vial 冻存供应 附 COA 与鉴定报告
HighQC™ Human (Parental) Head And Neck Cancer Stem Cell 产品信息表
产品名称HighQC™ Human (Parental) Head And Neck Cancer Stem Cell
中文名称请咨询
Cat No. 目录编号ABC-SC0132
Product Type 产品类型Cancer Stem Cells（肿瘤干细胞）
Cell Type 细胞类型Stem Cell
Species 物种Human
组织来源Head And Neck
培养条件37 ℃, 5% CO2
推荐培养基Culture Medium（基础培养基 + 血清 + 生长添加物），可随细胞一同订购
规格1 vial
形态与鉴定贴壁生长；随货提供免疫荧光鉴定图与阳性率数据（详见技术文档）
代次P1 – P3
质量控制无菌检测、支原体检测阴性；复苏后活率 ≥ 90%，随货提供 COA 与鉴定报告
储存条件Liquid Nitrogen
价格价格请询价

适用研究领域

  • 细胞功能与机制研究：目标细胞的基础表型、信号通路与基因功能验证。
  • 共培养与体外模型构建：与免疫细胞、内皮细胞或基质细胞共培养，构建体外微环境模型。
  • 疾病机制研究：炎症、纤维化、代谢与退行性改变相关表型与标志物检测。
  • 药物筛选与毒性评价：化合物处理后的活性、凋亡、氧化应激与细胞因子释放检测。

推荐培养基与添加物

  • Culture Medium（货号 ABC-TM-012）：配套基础培养基 + 血清 + 生长添加物，随细胞一同订购可保证批间一致。
  • 包被基质：按细胞类型选择多聚-D-赖氨酸（PDL）、层粘连蛋白或明胶，建议提前包被并晾干。
  • 冻存液：推荐使用无血清冻存液或含 10% DMSO 的完全培养基，程序降温后转入液氮。
  • 相关配件：细胞培养基与试剂产品线提供培养基、生长因子与细胞生物学试剂配套选择。

操作要点

  • 复苏建议 37 ℃ 水浴快速解冻（1 – 2 分钟内完成），立即转入预温培养基，离心去除冻存保护剂后接种。
  • 接种密度按细胞类型调整，贴壁前 24 小时避免晃动培养瓶，具体密度见随货说明书。
  • 每 2 – 3 天换液一次，汇合度约 80% 时传代；使用胰酶替代物或 0.05% 胰酶-EDTA 短时消化，避免过度消化。
  • 建议在低代次内完成实验，并定期以标志物免疫荧光复核细胞身份。

本产品仅限科研使用

本产品不用于临床诊断或治疗用途。如需用于体外诊断方向的细胞原料或需要特定供体资质与文件，请联系我们确认可行性与合规要求。

HighQC™ Human (Parental) Head And Neck Cancer Stem Cell 技术文档清单
文档名称类型语言说明
产品说明书（Product Manual） PDF 中 / 英 含复苏、接种、换液、传代与冻存操作流程
质检报告（COA） PDF 英 按批次出具，随货提供
细胞鉴定报告 PDF 英 免疫荧光鉴定图与阳性率数据
无菌与支原体检测报告 PDF 英 放行检测结果与检测方法说明
供体信息与伦理声明 PDF 英 供体年龄 / 性别 / 种族信息与知情同意说明
技术文档随货提供，如需电子版请联系我们。 前往资源中心了解文档体系 →

优先确认水浴解冻是否在 1 – 2 分钟内完成、冻存保护剂是否已离心去除，以及培养器皿是否已按要求包被。其次检查培养基是否预温至 37 ℃、接种后 24 小时内是否移动过培养瓶。若贴壁率仍低于 70%，请提供复苏记录与显微照片，我们协助复核批次情况。

原代细胞在体外培养中会随代次增加出现形态变化，属常见现象。若在早期代次即出现明显异常，通常与接种密度过高、血清批次差异或传代消化过度有关。建议降低接种密度、统一血清批次，并在汇合度 80% 左右及时传代。

收到后请先核对冻存管外观与干冰余量，若仍有干冰可暂存 −80 ℃，并尽快转入液氮气相长期保存；若干冰已完全升华，建议直接复苏使用或按说明书转入液氮。避免在 −80 ℃ 与液氮之间反复转移，也不要在 −20 ℃ 存放细胞。

每批细胞随货提供鉴定报告，含标志物免疫荧光图像与阳性率数据。建议实验前自行复核标志物表达，如需额外标志物检测或 STR 鉴定，可在询价时说明。

提供。常规规格见产品信息表，可申请大包装；特定物种、组织来源或疾病模型来源的定制分离服务按项目评估周期与报价。请在询价表单中注明细胞类型、物种、预计用量与标志物要求。

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需要其他物种或系统的细胞产品？

HighQC™ Human (Parental) Head And Neck Cancer Stem Cell 之外，我们还提供 Mouse、Rat、Canine 等物种的神经、免疫、血液、消化与呼吸系统产品，以及多种包装规格。请告知需求，我们提供对应报价。

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