Cell Counting Kit (CCK-8) CCK-8

The Cell Counting Kit-8, also called CCK-8, is an upgrade version of MTT. It is a rapid, highly sensitive tool for cell proliferation and toxicity assay.

The Cell Counting Kit-8, also known as CCK-8, is a rapid, highly sensitive assay for cell proliferation and cytotoxicity based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonylphenyl)-2H-tetrazolium monosodium salt). An upgraded version of MTT, WST-8 works by reducing it to a highly water-soluble orange-yellow formazan product (formazan) in the presence of an electron coupling reagent. The intensity of the color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The OD value is measured at a wavelength of 450 nm using a microplate reader, which indirectly reflects the number of viable cells.

CCK-8 is commonly used in biomedical research to assess cell proliferation, viability, and cytotoxicity in vitro. It can also evaluate antiviral activity of potent compounds against virus infection. The CCK-8 assay has a wide range of applications, including drug screening, cell proliferation assays, cytotoxicity assays, tumor drug sensitivity testing, and biological factor activity assays.