Caspase-1 is a protease regulating apoptosis and cytokine activation, detectable via YVAD-pNA substrate hydrolysis with 405 nm absorbance signal.
Caspases are a family of proteases involved in apoptosis, encompassing over ten members. Caspase-1 is the only caspase capable of cleaving IL-1b and IL-18 precursors to produce active cytokines. Caspase-11 cleaves the 45kD caspase-1 precursor protein to produce 20kD and 10kD fragments, which can form heterodimers and further tetramers. This tetramer possesses protease activity. Caspase-1 regulates apoptosis by cleaving Bcl-XL and modulates cytokine-mediated immune inflammatory responses by cleaving cytokine precursors. The assay is based on the specific hydrolysis of the peptide substrate Tyr-Val-Ala-Asp-p-nitroanilide (YVAD-pNA) by caspase-1. The released free nitroanilide pNA has a maximum absorbance at 405 nm.
Caspase-1 Colorimetric Assay Kit provides a convenient and simple way for detecting the YVAD-dependent caspase activity.
| 产品名称 | Caspase-1 Colorimetric Assay Kit |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0072 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 20 T, 48 T, 96 T |
| 储存条件 | PartⅠ in 2-8℃, PartⅡ in -20℃. |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
