The JC-10 Mitochondrial Membrane Potential Assay Kit use JC-10, an upgraded fluorescent probe to detect changes in mitochondrial membrane potential.
JC-10 is a mitochondrial membrane potential–sensitive fluorescent probe and an improved alternative to JC-1 for detecting changes in mitochondrial membrane potential (ΔΨm). Although JC-1 is widely used, its poor water solubility can cause precipitation in aqueous buffers even at low concentrations, limiting its application. JC-10 exhibits enhanced water solubility and can therefore replace JC-1 in experiments requiring higher dye concentrations. While JC-10 shows superior performance in some cell lines, its effectiveness remains highly cell-type dependent.
In healthy cells, JC-10 selectively accumulates in the mitochondrial matrix and forms reversible aggregates that emit red fluorescence (Ex = 540 nm, Em = 590 nm). When mitochondrial membrane potential decreases or collapses, JC-10 dissociates into monomers that localize in the cytoplasm and emit green fluorescence (Ex = 490 nm, Em = 525 nm).
| 产品名称 | JC-10 Mitochondrial Membrane Potential Assay Kit |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0060 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 100 T |
| 储存条件 | -20℃, protect from light, in dry enviornment, avoid repeated freezing and thawing. |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
