G418 Sulfate (Geneticin)

G418 Sulfate (Geneticin) by ImmunoClone is an aminoglycoside antibiotic for selecting eukaryotic and prokaryotic cells expressing neomycin resistance.

G418 Sulfate (Geneticin®) is an aminoglycoside antibiotic that inhibits protein synthesis by binding to the 80S ribosomal subunit, leading to misreading of mRNA and termination of translation in eukaryotic and prokaryotic cells. It is widely utilized as a selective agent for cells engineered to express the neomycin resistance gene (neo⁺), enabling stable cell line development, CRISPR-Cas9-edited clone selection, and maintenance of genetically modified eukaryotic cells. With broad-spectrum activity against bacteria, yeast, fungi, protozoa, and mammalian cells, G418 is effective in both in vitro and in vivo applications. Its high solubility and stability make it suitable for long-term selection experiments, transgenic model generation, and high-throughput screening in genetic engineering and biomedical research.

G418 Sulfate (Geneticin®) is widely used for the selection and maintenance of eukaryotic and prokaryotic cells expressing the neomycin resistance gene (neo⁺). It enables efficient establishment of stable cell lines, CRISPR-Cas9-edited clones, and genetically engineered microbial strains. This antibiotic is critical in selecting transgenic organisms, validating gene function, and conducting high-throughput genetic screens. With high solubility and stability, it supports short- and long-term selection in mammalian, yeast, bacterial, and plant cell cultures. G418 is essential in biomedical research, drug discovery, and the development of recombinant protein production systems.

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