Annexin V-Fluor 488/7-AAD Apoptosis Detection Kit

The Annexin V-Fluor 488/7-AAD Cell Apoptosis Detection Kit can be used to distinguish early apoptotic cells from late apoptotic or necrotic cells.

The Annexin V–Fluor 488/7-AAD Cell Apoptosis Detection Kit is designed to detect early apoptosis using Fluor 488–labeled Annexin V as a fluorescent probe. Detection can be performed by flow cytometry, fluorescence microscopy, or other fluorescence-based analytical instruments. In viable cells, phosphatidylserine (PS) is localized on the inner leaflet of the plasma membrane. During early apoptosis, PS is translocated to the outer leaflet and becomes exposed to the extracellular environment. Annexin V is a 35–36 kDa Ca²⁺-dependent phospholipid-binding protein that binds PS with high affinity, enabling selective labeling of early apoptotic cells. 7-AAD is a nucleic acid dye with fluorescence properties similar to propidium iodide but with a narrower emission spectrum, reducing spectral overlap in multicolor assays.

The Annexin V-Fluor 488/7-AAD Apoptosis Detection Kit is developed to detect apoptosis in both suspension and adherent cells and is compatible with flow cytometry, fluorescence microscopy, and other fluorescence-based analytical platforms. It cannot penetrate intact cell membranes but readily enters cells with compromised membrane integrity and binds DNA. When used together, cells can be classified as Annexin V⁻/7-AAD⁻ (live), Annexin V⁺/7-AAD⁻ (early apoptotic), and Annexin V⁺/7-AAD⁺ (late apoptotic or necrotic).