Puromycin

Puromycin by ImmunoClone is an antibiotic for protein synthesis inhibition and stable cell line selection in eukaryotic and prokaryotic systems.

Puromycin is a potent aminonucleoside antibiotic that inhibits protein synthesis by irreversibly binding to the 60S ribosomal subunit, causing premature chain termination during translation. It is widely used as a selective agent in molecular and cellular biology for the establishment of stable cell lines expressing resistance genes, such as pac / puroR. With rapid activity across prokaryotic and eukaryotic systems, puromycin effectively eliminates non-transfected cells within 1–7 days. It is also employed in CRISPR screening, antibiotic selection in transgenic models, and studies of protein synthesis dynamics. Available in powder or solution form, puromycin demonstrates high stability and consistent performance in both in vitro and in vivo applications.

Puromycin is widely used for selecting genetically modified eukaryotic and prokaryotic cells based on pac/puroR resistance. It enables efficient establishment of stable cell lines, CRISPR-edited pools, and transgenic models by eliminating non-transformed cells within days. This antibiotic is also applied in protein synthesis inhibition studies, high-throughput screening, and viral transduction validation. Suitable for both in vitro and in vivo research, puromycin selection ensures rapid and reliable results in molecular biology and therapeutic development.

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