hESC/iPSC Y27632 Supplement

Y27632 Supplement by ImmunoClone enhances hESC/iPSC survival by inhibiting ROCK and reducing apoptosis during culture, passaging, and cryopreservation.

hESC/iPSC Y27632 Supplement is a selective, ATP-competitive inhibitor of ROCK-I and ROCK-II, specifically designed to enhance the survival and efficiency of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) during critical culture processes. By inhibiting Rho-associated kinase (ROCK) signaling pathway activation triggered by cell dissociation, it effectively reduces apoptosis and improves cell viability after passaging, freezing, and thawing. This supplement is essential for single-cell cloning, reaggregation culture, and enhancing attachment post-cryopreservation. It increases plating efficiency, supports clonal expansion, and improves overall culture robustness, making it a vital tool for regenerative medicine research, organoid development, and large-scale stem cell production.

The hESC/iPSC Y27632 Supplement is specifically formulated to significantly enhance the survival and cloning efficiency of human pluripotent stem cells during critical manipulation stages. It is essential for applications involving single-cell passaging, cryopreservation, thawing, and genetic modification of hESCs and iPSCs. By inhibiting ROCK-mediated apoptosis, this supplement reduces cell death and improves recovery after dissociation, supporting robust clonal expansion and high-density culture. It is widely used in regenerative medicine research, organoid formation, directed differentiation protocols, and large-scale production of clinically relevant stem cell lines. Its consistent performance helps maintain genomic stability and pluripotency in challenging culture conditions.