Caspase-8 is a cysteine protease. The assay is based on the specific peptide substrate Ile-Glu-Thr-Asp-p-nitroanilide (IETD-pNA) hydrolysis by caspase-8.
Caspases are a family of proteases involved in apoptosis, encompassing over ten members. Caspase-8, also known as FLICE, MACH, or Mch5, typically exists as a zymogen and is activated during apoptosis. It is considered an upstream caspase in the apoptotic pathway. During Fas receptor- and TNFR-1-mediated apoptosis, caspase-8 is activated to form dimers, which in turn activate downstream caspases 4, 6, 9, and 10. The assay is based on the specific hydrolysis of the peptide substrate Ile-Glu-Thr-Asp-p-nitroanilide (IETD-pNA) by caspase-8. The released free nitroanilide (pNA) has a maximum absorbance at 405 nm.
Caspase-8 Colorimetric Assay Kit provides a convenient and simple way for detecting the IETD-dependent caspase activity.
| 产品名称 | Caspase-8 Colorimetric Assay Kit |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0078 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 20 T, 48 T, 96 T |
| 储存条件 | PartⅠ in 2-8℃, PartⅡ in -20℃. |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
