Caspase-9 can initiate apoptosis. The assay is based on the specific peptide substrate LEHD-pNA (Leu-Glu-His-Asp-p-nitroanilide) hydrolysis by caspase-9.
Caspase-9, also known as ICE-LAP6 or Mch6, forms a complex with cytochrome c and Apaf1, leading to activation. This complex further activates caspase-3, a key enzyme in apoptosis, thereby triggering the apoptotic cascade. Caspase-9 is a crucial upstream caspase in apoptotic signal transduction, and its activation can be regulated by phosphorylation. The assay is based on the specific hydrolysis of the peptide substrate LEHD-pNA (Leu-Glu-His-Asp-p-nitroanilide) by caspase-9. The released free nitroanilide pNA has a maximum absorbance at 405 nm.
Caspase-9 Colorimetric Assay Kit provides a convenient and simple way for detecting the LEHD-dependent caspase activity.
| 产品名称 | Caspase-9 Colorimetric Assay Kit |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0079 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 20 T, 48 T, 96 T |
| 储存条件 | PartⅠ in 2-8℃, PartⅡ in -20℃. |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
