The Annexin V-FITC/7-AAD Cell Apoptosis Detection Kit can be used to distinguish early apoptotic cells from late apoptotic or necrotic cells.
The kit detects early apoptotic cells using FITC-labeled Annexin V and can be analyzed by flow cytometry or fluorescence microscopy. In viable cells, phosphatidylserine (PS) is on the inner membrane. During early apoptosis, PS moves to the outer membrane. Annexin V is a 35–36 kDa Ca²⁺-dependent phospholipid-binding protein that binds PS with high affinity.
The kit also includes 7-aminoactinomycin D (7-AAD) to identify late apoptotic or necrotic cells. 7-AAD is a DNA dye that cannot penetrate live or early apoptotic cells but stains late apoptotic or necrotic cells. When used together, live cells are Annexin V⁻/7-AAD⁻, early apoptotic cells are Annexin V⁺/7-AAD⁻, and late apoptotic or necrotic cells exhibit double-positive staining (Annexin V⁺/7-AAD⁺).
| 产品名称 | Annexin V-FITC/7-AAD |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-BR0046 |
| Product Type 产品类型 | Cell Biology Reagents（细胞生物学试剂） |
| 规格 | 20 T, 50 T, 100 T |
| 储存条件 | 4℃, protect from light, do not freeze. |
| 价格 | 价格请询价 |
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复溶、稀释、储存与使用操作说明 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 安全数据表（MSDS） | 中 / 英 | 危险组分识别、防护措施与应急处置 |
