HighQC™ Mouse Hepatocytes Stem Cells are liver-resident bipotent stem cells derived from healthy adult C57BL/6 mice. These cells reside in the canals of Hering, a transitional zone between hepatocytes and bile ducts, and possess the capacity to differentiate into both hepatocytes and cholangiocytes under defined culture conditions. These cells exhibit small, oval-shaped morphology and adhere to plastic culture surfaces, forming clonogenic colonies in vitro with sustained in vitro expansion potential. At the undifferentiated stage, they express characteristic markers such as EpCAM, CD133, and CK19. This stem cell source offers a consistent and faithful model for liver progenitor biology. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from mycoplasma, fungi, yeast, and bacteria.
HighQC™ Mouse Hepatocyte Stem Cells serve as a powerful in vitro tool to study liver regeneration, biliary differentiation, and progenitor-driven repair mechanisms. They are well-suited for investigations into oval cell activation following liver injury, exploration of signaling pathways like Wnt and Notch in lineage fate decisions, and modeling chronic liver conditions, including fibrosis and cirrhosis, in mouse systems. Researchers also use them for preclinical drug screening, hepatotoxicity assays, and probing the molecular switches guiding hepatocyte vs. cholangiocyte differentiation.
| Growth Mode | Adherent |
|---|---|
| Shipping Info | Dry Ice |
| Biosafety Level | 1 |
| Disease Model | Normal |
| Synonyms | HighQC™ Mouse Hepatocytes Stem Cell, Mouse Hepatic Progenitor Cells, mHepSCs |
| 产品名称 | HighQC™ Mouse Hepatocytes Stem Cell |
|---|---|
| 中文名称 | 请咨询 |
| Cat No. 目录编号 | ABC-SC0041 |
| Product Type 产品类型 | Stem Cells（干细胞） |
| Cell Type 细胞类型 | Stem Cell |
| Species 物种 | Mouse |
| 组织来源 | Liver |
| 培养条件 | 37 ℃, 5% CO2 |
| 推荐培养基 | Culture Medium（基础培养基 + 血清 + 生长添加物），可随细胞一同订购 |
| 规格 | 1 vial |
| 形态与鉴定 | 贴壁生长；随货提供免疫荧光鉴定图与阳性率数据（详见技术文档） |
| 代次 | P1 – P3 |
| 质量控制 | 无菌检测、支原体检测阴性；复苏后活率 ≥ 90%，随货提供 COA 与鉴定报告 |
| 储存条件 | Liquid Nitrogen |
| 价格 | 价格请询价 |
本产品不用于临床诊断或治疗用途。如需用于体外诊断方向的细胞原料或需要特定供体资质与文件，请联系我们确认可行性与合规要求。
| 文档名称 | 类型 | 语言 | 说明 |
|---|---|---|---|
| 产品说明书（Product Manual） | 中 / 英 | 含复苏、接种、换液、传代与冻存操作流程 | |
| 质检报告（COA） | 英 | 按批次出具，随货提供 | |
| 细胞鉴定报告 | 英 | 免疫荧光鉴定图与阳性率数据 | |
| 无菌与支原体检测报告 | 英 | 放行检测结果与检测方法说明 | |
| 供体信息与伦理声明 | 英 | 供体年龄 / 性别 / 种族信息与知情同意说明 |
优先确认水浴解冻是否在 1 – 2 分钟内完成、冻存保护剂是否已离心去除，以及培养器皿是否已按要求包被。其次检查培养基是否预温至 37 ℃、接种后 24 小时内是否移动过培养瓶。若贴壁率仍低于 70%，请提供复苏记录与显微照片，我们协助复核批次情况。
原代细胞在体外培养中会随代次增加出现形态变化，属常见现象。若在早期代次即出现明显异常，通常与接种密度过高、血清批次差异或传代消化过度有关。建议降低接种密度、统一血清批次，并在汇合度 80% 左右及时传代。
收到后请先核对冻存管外观与干冰余量，若仍有干冰可暂存 −80 ℃，并尽快转入液氮气相长期保存；若干冰已完全升华，建议直接复苏使用或按说明书转入液氮。避免在 −80 ℃ 与液氮之间反复转移，也不要在 −20 ℃ 存放细胞。
每批细胞随货提供鉴定报告，含标志物免疫荧光图像与阳性率数据。建议实验前自行复核标志物表达，如需额外标志物检测或 STR 鉴定，可在询价时说明。
提供。常规规格见产品信息表，可申请大包装；特定物种、组织来源或疾病模型来源的定制分离服务按项目评估周期与报价。请在询价表单中注明细胞类型、物种、预计用量与标志物要求。
